ea0013oc19 | Novartis Basic Endocrinology Award | SFEBES2007
MacKenzie Scott
, Dewar Deborah
, Stewart William
, Fraser Robert
, Connell John
, Davies Eleanor
Previous studies investigating steroidogenic enzymes in the brain have often focused on only a few genes at a time, using animal models or pooled human RNA to investigate their expression. For this study, we obtained RNA from individual archived human brain tissue samples in order to analyse the transcription of twelve genes involved with steroid biosynthesis and metabolism within the cerebellum and hippocampus.We developed realtime RT-PCR assays for twe...